@article{Brechka_Bondarenko_Korenieva_Laryanovskaya_Morozenko_Kozar_Ashukina_Leontieva_Shcherbak_Ivannikova_2020, title={DYNAMICS OF GLYCOSAMINOGLYCANS CONTENT IN THE TESTES OF RATS AFTER ADMINISTRATION OF NATURAL HERBAL PRODUCTS*}, volume={72}, url={https://www.jpep.endocrinology.org.ua/index.php/1/article/view/149}, DOI={10.21856/j-PEP.2020.2.13}, abstractNote={<p>It is possible that testicular dysfunctions, including idiopathic infertility in men, may be caused by changes<br>in connective tissue, which are characterized by disorders of its metabolism, the content of collagen and glycosaminoglycans<br>(GAGs). Such patients quite often have the anomalies of the genital system. It is the anomalies<br>in the development of connective tissue structures of the venous wall and not compensated state of the valve<br>apparatus that lead to varicocele. The prevalence of varicocele among infertile patients reaches 40 %, whereas<br>male population in general has this disease only in 15 % of cases. Changes in GAG content may reflect the functional<br>status of the semen, which ensures normal spermatogenesis. Therefore, the study of this indicator can be<br>used to explain the mechanisms of infertility and to find ways to correct both hypo- and infertile conditions in<br>men.<br>Therefore, the aim of our work was to study the content of glycosaminoglycans in the samples of the<br>testes affected with serotonin hydrochloride and correction of this condition with such drugs as Tribestan,<br>Chondroitin sulfate and Bioglobin-U.<br>Materials. A s erotonin l esion m odel w as u sed t o o btain t he p athology o f t he t estes; r ats w ere g iven<br>Serotonin hydrochloride (Alfa Aesar®) for 14 days, subcutaneously at a dose of 5 mg/kg of body weight) and on<br>the background of it was administered Chondroitin sulfate at a dose of 60 mg/kg of body weight, Bioglobin-U<br>at a dose of 200 μl/kg of body weight and Tribestan — 60 mg/kg of body weight. In this work we performed<br>histochemical studies of rat testes. For the histochemical study of GAGs content in the samples of testes, they<br>were fixed in 10 % formalin solution, dehydrated in alcohols of increasing strength and poured into paraffin.<br>Detection of GAGs on sections was a metachromatic reaction by staining with toluidine blue at pH 2.5: there<br>occurred a change in color from blue to purple color of various degrees of saturation due to the combination of<br>a high degree of dissociation of the acid groups of the substrate.<br>Results. It was shown the histochemical methods and metachromatic reaction with toluidine blue showed<br>that intact sexually mature male rats’ testes had a sufficient number of glycosaminoglycans, which was visualized<br>in the tape of the spermatogenic epithelium and small areas of metachromaticemia. The results of<br>histochemical study of glycosaminoglycans content in animals with induced gonadopathy by administration<br>of serotonin hydrochloride indicated glycosaminoglycans deficiency. Administration of Chondroitin sulfate,<br>«Bioglobin-U» and Tribestan during serotonin lesions of the testes significantly increased glycosaminoglycans<br>in the tubules and intercranial stroma.</p>}, number={2}, journal={Problems of Endocrine Pathology}, author={Brechka, N. M. and Bondarenko, V. O. and Korenieva, Ye. M. and Laryanovskaya, Yu. B. and Morozenko, D. V. and Kozar, V. V. and Ashukina, N. A. and Leontieva, F. S. and Shcherbak, E. V. and Ivannikova, S. V.}, year={2020}, month={May}, pages={104-110} }