AGE CHARACTERISTICS OF MORPHOGENESIS OF DIABETIC MYOPATHIES
DOI:
https://doi.org/10.21856/j-PEP.2020.4.15Keywords:
masticatory muscle, neuromuscular junction, streptozotocin-induced diabetes mellitusAbstract
One of the complications of diabetes mellitus (DM) is diabetic myopathy, which is diagnosed in 88% of patients. Therefore, the purpose of our study was to establish the age characteristics of histo-ultrastructural changes in the masticatory muscle of 2-month (immature) and 6-month (mature) rats with streptozotocin-induced diabetes mellitus (STD). It was induced by a single intraperitoneal injection of streptozotocin 7 mg/100 g body weight in 2-month animals, and 6 mg/100 g body weight in 6-month-old rats. It was found that on the 70th day of STD there was a muscle sequestration and focal aseptic inflammatory cell infiltrates in the masticatory muscle of 2-month-old rats, while in 6-month-old animals the fibrosis and focal lysis of individual muscle fibers (MF), sometimes their necrosis and partial replacement by connective tissue were noticed. Sonography revealed in 6-month-old rats partial necrosis of MF, karyorexis, subsarcolemic edema, fibrosis and lysis of myofibrils, destruction of mitochondria; 2-month animals had intracellular regenerative processes: formation of young mitochondria, appearance of numerous granules of glycogen in the sarcoplasm between myofibrils. Such changes occur against the background of the diabetic microangiopathy. Adult animals had a massive destruction of neuromuscular junctions (NMJs) and it leads to a decrease in the area of axon sprouting by 67% and is accompanied by partial destruction of neuromuscular synapses. In 2-month-old rats, there was also a decrease in the area of NMJs by 54%, while in some NMJs the reinnervation processes were noted due to collaterals from neighboring nerve fibers. Thus, diabetic myopathy is diagnosed in the masticatory muscle of SDM rats of different ages, which results from the development of diabetic microangiopathy and peripheral neuropathy. In adult animals, it leads to atrophy and partial sclerosis of MF of the masticatory muscle, and in immature animals, compensatory-restorative processes are observed in the NMJs and MF.
References
Tkachuk YL. Journal of Education, Health and Sport formerly Journal of Health Sciences 2016; 6(4): 375-384.
Zhurakivska OY, Mykulets TI, Dutchak UM, et al.World of medicine and biology 2018; 1: 126-130. doi:http://doi.org/10.26.724/2079-8334-2018-1-63-126-130.
Venojärvi M, Puhke R, Hämäläinen H, et al. Diabetes,Obesity and Metabolism 2005; 7: 745-754.
Krause MP, Riddell MC, Hawke TJ. Pediatr Diabetes 2011;12(4 Pt 1): 345-364. doi:
http://doi.org/10.1111/j.1399-5448.2010.00699.x.
Zhurakivska OYa, Grad AO, Popel SL, et al. World of medicine and biology 2017;3(61): 108-114. doi: http://doi.org/10.26724/2079-8334-2017-3-61-108-114.
Levitskiy VA, Atamanchuk OV. Journal of the Grodno State Medical University 2014;46: 70-74.
Slavnov AA, Dolgikh VT. General Reanimatology 2014; 10(4): 37-43. doi: http://doi.org/10.15360/1813-9779.2014.10.4.37-43.
Zishan Md. J Pharm Pharm Sci 2017; 7: 448-470, available at: https://doi.org/10.20959/wjpps20175-9135.
Stepanova Z, Maksyukov S, Skorobogach M, et al. Medical news of the North Caucasus 2017; 12(20): 34-42,
available at: https://doi.org/10.14300/mnnc.2017.12051.
Reddy VS, Jakhotia S, Reddy PY, Reddy GB. IUBMB Life 2015; 67(4): 291-299.
Kuryliszyn-Moskal A, Zarzycki W, Dubicki A, et al. Adv Med Sci 2017; 62(2): 368-373.
Madonna R, Balistreri CR, Geng YJ, De Caterina R. Vascul Pharmacol 2017; 90: 1-7. doi: http://doi.org/10.1016/j.vph.2017.01.004.
Yushkov PV, Opalenov KV. Diabetes mellitus 2001; 4(1):53-56.
Leung WK, Gao L, Siu PM, Lai CW. Life Sci 2016; 166:121-130. doi: http://doi.org/10.1016/j.lfs.2016.10.015.
Tokaruk NS. Topical problems of modern medicine:Bull. of the Ukr. Med. Dent. Acad 2016; 16(1): 249-255.
Garcia CC, Potian JG, Hognason K, et al. Am J Physiol Endocrinol Metab 2012; 303: 551-561. doi: http://doi.org/10.1152/ajpendo.00622.2011.
Marques MJ, Neto HS. The Anat Record 2002; 267: 112-119.
Fahim MA, Hasan MY, Alshuaib WB. J Appl Physiol 2000; 89: 2235-2240. doi:http://doi.org/10.1152/jappl.2000.89.6.2235.
ISSN 
ISSN 
