THE ROLE OF ACID SPHINGOMYELINASE IN INSULIN-INDUCED GLUCOSE UPTAKE VIOLATIONS BY MUSCLE TISSUE IN OLD AGE

Authors

  • Babenko N. A. Institute of Biology, V. Karazin Kharkiv National University
  • Timofiychuk O. A. Institute of Biology, V. Karazin Kharkiv National University

DOI:

https://doi.org/10.21856/j-PEP.2013.3.10

Keywords:

ceramide, acid sphingomyelinase, imipramine, insulin resistance, aging

Abstract

It was study the possibility of correction of violations of the insulin-induced glucose uptake by
muscle tissue of older rats by inhibiting acid sphingomyelinase (aSMase) with imipramine. Found
that in muscle tissue 24-month-old rats, there are an accumulation of ceramide, weight sphingomyelin
(SM) and the growth of the ratio of ceramide/SM compared to 3-month-old rats. The introduction
of imipramine old animal, like the introduction of the drug in the incubation medium of the extensor
digitorum longus, induces a decrease in the level of ceramide accumulation of SM and drops of the
ratio of ceramide/SM compared with the control, which may be due to the ability of the drug to
inhibit the activity of aSMase. Effect of imipramine in vitro accompanied by improved insulin-induced
glucose uptake by muscle tissue of old rats. It is suggested that aSMase does an important role in the
modulation of insulin signaling in target tissues in old age through the production of ceramide.

References

Rustad JK, Musselman DL, Nemeroff CB. Psychoneuroendocrinology 2011; 36:1276-1286.

Wagner J, Allen NA, Swalley LM, et al. Diabetes Res Clin Pract 2009; 86(2):96-103.

Wieser V, Moschen AR, Tilg H. Arch Immunol Ther Exp 2013; 61(2):119-125.

Wei Y, Chen K, Whaley-Connell AT, et al. Am J Physiol Regul Integr Comp Physiol 2008; 294:R673-R680.

Chen YC, Lin WW, Chen YJ, et al. Mediators Inflammation 2010. doi: 10.1155/2010/573594.

Kornhuber J, Medlin A, Bleich S, et al. J Neural Transm 2005; 112:1583-1590.

Samad F, Hester KD, Yang G, et al. J Diabetes 2006; 55:2579-2587.

Gorski J. Diabetes 2012; 61:3081-3083.

Babenko NA, Harchenko VS. Problemy endokrynnoi’ patologii’ 2009; 1:37-43.

Jenkins RW, Canals D, Idkowiak-Baldys J, et al. Ibid 2010; 285(46):35706-35718.

Garkavenko VV, Storozhenko GV, Krasnikova ON, Babenko NA. Inter J Physiol Pathophysiol 2012; 3(3):281-286.

Bligh EG, Dyer WJ. Can J Biochem Physiol 1959; 37(8):911-917.

Findlej DB. Biologicheskie membrany. Metody, Moskva , 1990:424 p.

Babenko NA, Kharchenko VS. Biochemistry (Moscow) 2012; 77(2):180-186.

Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. J Biol Chem 1951; 193:365-375.

Babenko N, Shakhova E. Experim Gerontol 2006; 41(1):32-39.

Deevska GM, Rozenova KA, Giltiay NV, et al. J Biol Chem 2009; 284(13):8359-8368.

Herschkovitz A, Liu YF, Ilan E, et al. J Biol Chem 2007; 282(25):18018-18027.

Hofmeister R, Wiegmann K, Korherr C, et al. J Biol Chem 1997; 272:27730-27736.

Henkes LE, Sullivan BT, Lynch MP, et al. Proc Natl Acad Sci USA 2008; 105(22):7670-7675.

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Published

2013-10-10

How to Cite

Babenko, N. A., & Timofiychuk, O. A. (2013). THE ROLE OF ACID SPHINGOMYELINASE IN INSULIN-INDUCED GLUCOSE UPTAKE VIOLATIONS BY MUSCLE TISSUE IN OLD AGE. Problems of Endocrine Pathology, 45(3), 75-81. https://doi.org/10.21856/j-PEP.2013.3.10