FUNCTIONAL RELEVANCE OF THE INTERLEUKINE-6 GENE POLYMORPHISM RS1800795 IN PATIENTS WITH T YPE 2 DIABETES OF THE EAST UKRAINIAN POPULATION
Keywords:ype 2 diabetes mellitus, interleukin-6 gene, polymorphism
Single nucleotide polymorphisms (SNPs) of the interleukin-6 (IL6) gene have recently been studied actively in various populations to assess its contribution towards multifactorial pathologies formation, including diabetes mellitus (DM). The aim of the study was to evaluate the functional relevance of the IL6 gene SNP -174G/C (rs1800795) in patients with type 2 DM in the East Ukrainian Caucasoid population. Methods. The study included a total of 60 patients with a diagnosis of T2D (M/F: 34/26, age 53.79 ± 1.22 yrs, T2D duration 6.08 ± 0.64 yrs, body mass index (BMI) 32,68 ± 0,77 kg/m2, HbA1c 7.80 ± 0.19 %). In all the IL6 SNP -174G > С was determined by polymerase chain reaction — restriction fragment length polymorphism method with endonuclease Hin1II (NlaIII). All patients signed an informed consent for clinical, biochemical and genetic studies and the protocols were approved by the institutional review board of SI «V. Danilevsky Institute of Endocrine Pathology Problems of NAMS of Ukraine». Subjects from study were all Caucasoids and residents of the East Ukraine. Statistical analysis was performed with Unpaired Student’s t and U test. Data are expressed as median 25th-75th percentile. Results. It was found the functional relevance of the IL6 gene SNP -174G/C in type 2 diabetic patients of the East Ukrainian population, and the GG-genotype (vs CC) was associated with higher levels of IL-6 (8.36 ng/L (6.86–10.86) vs 5.67 ng/L (3.82–9.60), p = 0.049), TNF-α (6.80 μg/L (0.93–7.16) vs 1.18 μg/L (0.70–1.41), p = 0.043), reduced levels of high molecular weight adiponectin (1.18 mg/L (1.09–1.55) vs 2.53 mg/L (1.61–3.99), p = 0.006), and decreased activity of glutathione peroxidase (406.0 U/mmol Hb (362.0–487.0) vs 536.0 U/mmol Hb (428.0–623.5), p = 0.042). The data obtained under the analysis within the dominant model (GG vs CC+GC) regarding the levels of leptin (39.70 μg/l (24.30; 70.40) μg/l vs 53.45 μg/l (25.48; 94.60) μg/l, p = 0.051), indicates the possible obesogenic role of the C-allele in patients with type 2 DM in the studied population. Conclusion. We have found that the polymorphism IL6 -174G>С is functionally significant in type 2 diabetic patients of the East Ukrainian population. The prospects of the studies for clarification of the estimated polymorphism C-allele role in the modulation of «obesogenic» factors in type 2 diabetic patients of the East Ukrainian population, according to gender, are substantiated.
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