CHANGES OF mRNA GENE EXPRESSION LEVEL OF AIRE, DEAF1, FOXP3, CTLA4 AND IL-10 IN THE OFFSPRING OF RATS WITH EXPERIMENTAL GESTATIONAL DIABETES AND IN CONDITIONS OF INSULIN ORAL TOLERANCE FORMATION
DOI:
https://doi.org/10.21856/j-PEP.2016.3.06Keywords:
experimental gestational diabetes, mesenteric lymph nodes, Aire, Deaf1, FoxpAbstract
Using molecular genetic and immunofluorescence techniques it was investigated the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and Il-10 in the offspring of rats with experimental gestational diabetes and in conditions of insulin oral tolerance formation. To determine the level of mRNA it was studied gene RT-PCR and was performed in real time thermocycler CFX96 ™ RealTime PCR Detection Systems («Bio-Rad Laboratories, Inc.», USA). It was observed such violations of immunotolerance: AIRE gene repression, reduced mRNA levels of Deaf1 and the transcription factor Foxp3. Numbers of T-regulatory cells were decreased, which was confirmed by inhibition of gene expression cytokine Il-10 and the negative Ctla4 costimulatory molecule. Oral injection of insulin during first 2 weeks compensated these changes. It caused transcriptional activation of the gene AIRE, Deaf1, Foxp3, Ctla4 and Il-10, increased the number of Foxp3+ cells in mesenteric lymph nodes.
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