CHANGES OF mRNA GENE EXPRESSION LEVEL OF AIRE, DEAF1, FOXP3, CTLA4 AND IL-10 IN THE OFFSPRING OF RATS WITH EXPERIMENTAL GESTATIONAL DIABETES AND IN CONDITIONS OF INSULIN ORAL TOLERANCE FORMATION

Authors

  • Prozorova T. M. Zaporozhye state medical university, Zaporozhye
  • Kamyshnyy O. M. Zaporozhye state medical university, Zaporozhye

DOI:

https://doi.org/10.21856/j-PEP.2016.3.06

Keywords:

experimental gestational diabetes, mesenteric lymph nodes, Aire, Deaf1, Foxp

Abstract

Using molecular genetic and immunofluorescence techniques it was investigated the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and Il-10 in the offspring of rats with experimental gestational diabetes and in conditions of insulin oral tolerance formation. To determine the level of mRNA it was studied gene RT-PCR and was performed in real time thermocycler CFX96 ™ RealTime PCR Detection Systems («Bio-Rad Laboratories, Inc.», USA). It was observed such violations of immunotolerance: AIRE gene repression, reduced mRNA levels of Deaf1 and the transcription factor Foxp3. Numbers of T-regulatory cells were decreased, which was confirmed by inhibition of gene expression cytokine Il-10 and the negative Ctla4 costimulatory molecule. Oral injection of insulin during first 2 weeks compensated these changes. It caused transcriptional activation of the gene AIRE, Deaf1, Foxp3, Ctla4 and Il-10, increased the number of Foxp3+ cells in mesenteric lymph nodes.

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Published

2021-08-17

How to Cite

Prozorova, T. M., & Kamyshnyy, O. M. (2021). CHANGES OF mRNA GENE EXPRESSION LEVEL OF AIRE, DEAF1, FOXP3, CTLA4 AND IL-10 IN THE OFFSPRING OF RATS WITH EXPERIMENTAL GESTATIONAL DIABETES AND IN CONDITIONS OF INSULIN ORAL TOLERANCE FORMATION. Problems of Endocrine Pathology, 57(3), 50-59. https://doi.org/10.21856/j-PEP.2016.3.06