Expression of receptors of innate immunity by lymphocytes of pancreatic lymph nodes at experimental streptozotocin-induced diabetes and after introduction of metformin

Authors

  • Putilin D. A. Zaporizhzhia State Medical University, Zaporozhye, Ukraine
  • Kamyshnyi O. M. Zaporizhzhia State Medical University, Zaporozhye, Ukraine
  • Sukhomlinova I. Ye. Zaporizhzhia State Medical University, Zaporozhye, Ukraine
  • Kamyshna V. A. Zaporizhzhia State Medical University, Zaporozhye, Ukraine

DOI:

https://doi.org/10.21856/j-PEP.2017.1.07

Keywords:

pattern recognition receptor, pancreatic lymph nodes, innate immunity, diabetes mellitus

Abstract

The change of the expression of a variety of pattern-recognizing receptors: membrane and endosomal Toll-like receptors, cytoplasmic NOD-like receptors, viral RNA sensors such as the RIGlike receptors may play a critical role in the development of the type 1 diabetes mellitus (DM). The purpose of the thesis was to find out the pattern of distribution of TLR2+-, TLR4+-, NOD2+- and RIGI+-lymphocytes in pancreatic lymph nodes of rats with experimental streptozotocin-induced diabetes after introduction of metformin. The indirect immunofluorescence method with the use of monoclonal or polyclonal antibodies to the respective pattern-recognizing receptors was used to identify the immunopositive lymphocytes. The development of diabetes is accompanied by the change in the number of immunopositive lymphocytes, and the introduction of metformin to diabetic rats changes the number of lymphocytes and affects the density and concentration of receptors of innate immunity.

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Published

2017-02-20

How to Cite

Putilin, D. A., Kamyshnyi, O. M., Sukhomlinova, I. Y., & Kamyshna, V. A. (2017). Expression of receptors of innate immunity by lymphocytes of pancreatic lymph nodes at experimental streptozotocin-induced diabetes and after introduction of metformin . Problems of Endocrine Pathology, 59(1), 63-70. https://doi.org/10.21856/j-PEP.2017.1.07