Effect of natural origin medicines on prooxidant-antioxidant system in the prostate rats and spermatogenesis under conditions of the experimental prostatitis

Authors

  • Brechka N. M. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine
  • Bondarenko V. O. SI «V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine», Kharkiv, Ukraine
  • Morozenko D. V. National Pharmaceutical University, Kharkiv, Ukraine

DOI:

https://doi.org/10.21856/j-PEP.2019.3.13

Keywords:

non-bacterial prostatitis, inflammation, lipid peroxidation, antioxidant system, spermatogenesis, Chondroitin sulfate, placenta, Tribestan, «Prostaplant Forte»

Abstract

One of the pathogenic factors in all forms of man's infertility, including the chronic prostatitis as a result, leading to a violation of spermatogenesis, and to the overproduction of reactive oxygen species in semen, which is defined as oxidative stress. The accumulation of reactive oxygen species in sperm leads to damage to sperm membranes, their DNA fragmentation and decrease in their motility. In addition, there is an imbalance of the hormonal profile, which promote the development of man infertility with oxidative stress. The purpose of this work was investigation of prooxidant-antioxidant processes state in rats’ spermogram indicators under conditions of experimental prostatitis and its pharmacological correction by Chondroitin sulfate and its combination with Tribestan and placenta. Non-bacterial prostatitis have been caused by double rectal administration to male rats of 1 ml of the mixture based on a 10 % solution of dimexide in water and turpentine in a 4:1 ratio (model of turpentine prostatitis). Chondroitin sulfate, Chondroitin sulfate + Tribestan, Tribestan, Bioglobin-B, Prostaplant were administered for 14 days. The morphofunctional state of sperm cells and indicators represent of prooxidant-antioxidant processes state in the prostate homogenate have been studied: the content of diene conjugates, lipid hydroperoxides, products which reacts with thiobarbituric acid (TBA products) and reduced glutathione. Turpentine prostatitis is characterized by an intensification of lipid peroxidation processes and decrease of antioxidant protection processes, which negatively affects on the functional state of spermatozoa have been established. All studied medicines have a normalizing effect on the processes of lipid peroxidation and the functional state of spermatozoa. The study of the products of the prooxidant-antioxidant system in homogenates of the prostate gland and rats’ spermogram indicators showed the advantage of using Chondroitin sulfate in compared to other studied medicines.

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Published

2019-08-28

How to Cite

Brechka, N. M., Bondarenko, V. O., & Morozenko, D. V. (2019). Effect of natural origin medicines on prooxidant-antioxidant system in the prostate rats and spermatogenesis under conditions of the experimental prostatitis . Problems of Endocrine Pathology, 69(3), 98-105. https://doi.org/10.21856/j-PEP.2019.3.13

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