Effect of combined treatment with insulin and other hypoglycemic drugs on 5'amp-activated protein kinase activity in leucocytes of patients with diabetes mellitus

Authors

  • Pushkarev V. V. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine
  • Sokolova L. K. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine
  • Pushkarev V. M. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine
  • Belchina Y. B. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine
  • Vatseba T. S. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine
  • Tronko M. D. SI «V. P. Komisarenko Institute of Endocrinology and Metabolism of NAMS of Ukraine», Kiev, Ukraine

DOI:

https://doi.org/10.21856/j-PEP.2019.3.10

Keywords:

AMRK, leukocytes, type 2 diabetes, metformin, insulin, gliclazide MR, dapagliflozin

Abstract

The composition of leukocytes includes monocytes/macrophages and lymphocytes - cells, inflammation and infiltration of which is an important event in the pathogenesis of diabetes and related complications. The enzyme immunoassay method determined in leukocytes the level of threonine 172 phosphorylation (activation) of 5’adenosine monophosphate-activated protein kinase (AMPK), which controls the cell energy balance, in the combined treatment of patients with with insulin, metformin, gliklazidy MR and g, and the rats of heart and glyclaside MR and chromploidine, and ga; The level of threonine 172 phosphorylation (activation) of 5'-adenosine monophosphate-activated protein kinase (AMPK), controlling the energy balance of the cell, at combination treatment of patients with type 2 diabetes (T2D) with insulin, metformin, gliclazide and dapagliflozin was determined in blood cells by enzyme immunoassay. It has been shown that metformin drugs increase the activity of AMPK in the blood cells of patients with T2D more than 3-6 times compared with patients before treatment. Insulin and its analogue completely suppress AMPK activity, induced by metformin in the leucocytes of patients with T2D, which may indicate a decrease in the therapeutic effect of metformin. Gliclazide MR increases the activity of AMPK in blood mononuclear cells. In the presence of both metformin and gliclazide, the level of AMPK phosphorylation is reduced. The mechanism of activation of AMPK by gliclazide probably related to the effect of the latter on Ерас2А. Dapagliflozin increases the activity of AMPK and enhances the effect of metformin in the leucocytes of patients with T2D. AMPK activity in blood cells can serve as one of the indicators of the effectiveness of the hypoglycemic drugs action. The mechanisms of drug interaction and the consequences of their antagonism are discussed.

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Published

2019-08-28

How to Cite

Pushkarev, V. V., Sokolova, L. K., Pushkarev, V. M., Belchina, Y. B., Vatseba, T. S., & Tronko, M. D. (2019). Effect of combined treatment with insulin and other hypoglycemic drugs on 5’amp-activated protein kinase activity in leucocytes of patients with diabetes mellitus . Problems of Endocrine Pathology, 69(3), 74-82. https://doi.org/10.21856/j-PEP.2019.3.10

Issue

Section

CLINICAL ENDOCRINOLOGY

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