DYNAMICS OF GLYCOSAMINOGLYCANS CONTENT IN THE TESTES OF RATS AFTER ADMINISTRATION OF NATURAL HERBAL PRODUCTS*

Authors

  • Brechka N. M. SI «V. Danilevsky Institute for Endocrine Pathology Problems of NAMS of Ukraine», Kharkiv, Ukraine
  • Bondarenko V. O. SI «V. Danilevsky Institute for Endocrine Pathology Problems of NAMS of Ukraine», Kharkiv, Ukraine;
  • Korenieva Ye. M. SI «V. Danilevsky Institute for Endocrine Pathology Problems of NAMS of Ukraine», Kharkiv, Ukraine
  • Laryanovskaya Yu. B. National University of Pharmacy, Kharkiv, Ukraine
  • Morozenko D. V. National University of Pharmacy, Kharkiv, Ukraine
  • Kozar V. V. National University of Pharmacy, Kharkiv, Ukraine
  • Ashukina N. A. Sytenko Institute of Spine and Joint Pathology NAMS of Ukraine, Kharkiv, Ukraine
  • Leontieva F. S. Sytenko Institute of Spine and Joint Pathology NAMS of Ukraine, Kharkiv, Ukraine
  • Shcherbak E. V. Kharkiv State Zoo-Veterinary Academy, Kharkiv, Ukraine
  • Ivannikova S. V. Private Company «ALVIS-CLASS», clinico-diagnostic laboratory, Kharkiv, Ukraine

DOI:

https://doi.org/10.21856/j-PEP.2020.2.13

Keywords:

Chondroitin sulfate, Serotonin hydrochloride, varicocele, glycosaminoglycans, metachromatic reaction, sperm

Abstract

It is possible that testicular dysfunctions, including idiopathic infertility in men, may be caused by changes
in connective tissue, which are characterized by disorders of its metabolism, the content of collagen and glycosaminoglycans
(GAGs). Such patients quite often have the anomalies of the genital system. It is the anomalies
in the development of connective tissue structures of the venous wall and not compensated state of the valve
apparatus that lead to varicocele. The prevalence of varicocele among infertile patients reaches 40 %, whereas
male population in general has this disease only in 15 % of cases. Changes in GAG content may reflect the functional
status of the semen, which ensures normal spermatogenesis. Therefore, the study of this indicator can be
used to explain the mechanisms of infertility and to find ways to correct both hypo- and infertile conditions in
men.
Therefore, the aim of our work was to study the content of glycosaminoglycans in the samples of the
testes affected with serotonin hydrochloride and correction of this condition with such drugs as Tribestan,
Chondroitin sulfate and Bioglobin-U.
Materials. A s erotonin l esion m odel w as u sed t o o btain t he p athology o f t he t estes; r ats w ere g iven
Serotonin hydrochloride (Alfa Aesar®) for 14 days, subcutaneously at a dose of 5 mg/kg of body weight) and on
the background of it was administered Chondroitin sulfate at a dose of 60 mg/kg of body weight, Bioglobin-U
at a dose of 200 μl/kg of body weight and Tribestan — 60 mg/kg of body weight. In this work we performed
histochemical studies of rat testes. For the histochemical study of GAGs content in the samples of testes, they
were fixed in 10 % formalin solution, dehydrated in alcohols of increasing strength and poured into paraffin.
Detection of GAGs on sections was a metachromatic reaction by staining with toluidine blue at pH 2.5: there
occurred a change in color from blue to purple color of various degrees of saturation due to the combination of
a high degree of dissociation of the acid groups of the substrate.
Results. It was shown the histochemical methods and metachromatic reaction with toluidine blue showed
that intact sexually mature male rats’ testes had a sufficient number of glycosaminoglycans, which was visualized
in the tape of the spermatogenic epithelium and small areas of metachromaticemia. The results of
histochemical study of glycosaminoglycans content in animals with induced gonadopathy by administration
of serotonin hydrochloride indicated glycosaminoglycans deficiency. Administration of Chondroitin sulfate,
«Bioglobin-U» and Tribestan during serotonin lesions of the testes significantly increased glycosaminoglycans
in the tubules and intercranial stroma.

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Published

2021-06-30

How to Cite

Brechka, N. M. . ., Bondarenko, V. O. . ., Korenieva, Y. M. . ., Laryanovskaya, Y. B. . ., Morozenko, D. V. . ., Kozar, V. V. . ., Ashukina, . N. A. . ., Leontieva, . F. S. ., Shcherbak, E. V. . ., & Ivannikova, S. V. . (2021). DYNAMICS OF GLYCOSAMINOGLYCANS CONTENT IN THE TESTES OF RATS AFTER ADMINISTRATION OF NATURAL HERBAL PRODUCTS*. Problems of Endocrine Pathology, 72(2), 104-110. https://doi.org/10.21856/j-PEP.2020.2.13

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